Publications
Nature Biotechnology | 2023
Recording of cellular physiological histories along optically readable self-assembling protein chains
C. Linghu, B. An, M. Shpokayte, O. T. Celiker, N. Shmoel, R. Zhang, C. Zhang, D. Park, W. M. Park, S. Ramirez and E. S. Boyden
Summary: biological information over time, such as gene expression history, can be recorded onto the ordered structure of intracellular protein self-assembly for scalable readout in large cell populations.
Methods in Molecular Biology | 2022
Single-cell resolution optogenetics via expression of soma-targeted rhodopsins
C. Linghu, I. Chen, D. Tanese, V. Zampini and O. A. Shemesh
Summary: methods for temporally precise single-cell resolution optogenetics in tissue and in the living brain.
Cell | 2020 (featured in the cover image)
Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics
C. Linghu*, S. L. Johnson*, P. A. Valdes, O. A. Shemesh, W. M. Park, D. Park, K. D. Piatkevich, A. T. Wassie, Y. Liu, B. An, S. A. Barnes, O. T. Celiker, C.-C. Yao, C.-C. Yu, R. Wang, K. P. Adamala, M. F. Bear, A. E. Keating and E. S. Boyden (* co-first authors)
Summary: clustering fluorescent reporters at random points in cells enables many biological signals to be recorded at once.
Neuron | 2020
Precision calcium imaging of dense neural populations via a cell-body-targeted calcium indicator
O. A. Shemesh*, C. Linghu*, K. D. Piatkevich*, D. Goodwin, O. T. Celiker, H. J. Gritton, M. F. Romano, R. Gao, C.-C. Yu, H.-A. Tseng, S. Bensussen, S. Narayan, C. Yang, L. Freifeld, C. A. Siciliano, I. Gupta, J. Wang, N. Pak, Y. G. Yoon, J. F.P. Ullmann, B. Guner-Ataman, H. Noamany, Z. R. Sheinkopf, W. M. Park, S. Asano, A. E. Keating, J. S. Trimmer, J. Reimer, A. S. Tolias, M. F. Bear, K. M. Tye, X. Han, M. B. Ahrens and E. S. Boyden (* co-first authors)
Summary: restricting fluorescent reporters to the cell body reduces neuropil crosstalk, reduces artifactual spikes and correlations, and increases signal-to-noise ratio in calcium imaging of neural populations in vivo.
Nature Chemical Biology | 2018
A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
K. D. Piatkevich*, E. E. Jung*, C. Straub, C. Linghu, D. Park, H. J. Suk, D. R. Hochbaum, D. Goodwin, E. Pnevmatikakis, N. Pak, T. Kawashima, C. T. Yang, J. L. Rhoades, O. A. Shemesh, S. Asano, Y. G. Yoon, L. Freifeld, J. L. Saulnier, C. Riegler, F. Engert, T. Hughes, M. Drobizhev, B. Szabo, M. B. Ahrens, S. W. Flavell, B. L. Sabatini and E. S. Boyden (* co-first authors)
Summary: a robotic multidimensional screening system for mammalian cells enables rapid developments of better fluorescent proteins and better fluorescent voltage indicators.
Nature Neuroscience | 2017
Temporally precise single-cell resolution optogenetics
O. A. Shemesh *, D. Tanese*, V. Zampini*, C. Linghu, K. D. Piatkevich, E. Ronzitti, E. Papagiakoumou, E. S. Boyden+ and V. Emiliani+ (* co-first authors; + co-corresponding authors)
Summary: soma-targeted opsins, together with computer-generated holography systems, enable temporally precise, single-cell resolution optogenetics.
Chinese Physics B | 2013
Graphene applications in electronic and optoelectronic devices and circuits
H. Wu, C. Linghu, H. Lv and H. Qian
Summary: a review of how the unique properties of graphene could be utilized for better electronic and optoelectronic devices.
Proceedings of International Conference on Simulation of Semiconductor Processes and Devices | 2012
Compact model of graphene field effect transistors and its application in circuit simulation of RF mixer consisting of GFETs and CMOS
W. Zhu, C. Linghu, J. Zhang, L. Zhang and Z. Yu
Summary: modeling graphene-based transistors helps to design integrated circuits that contain both graphene-based transistors and conventional semiconductor transistors.